In this study, patchouli alcohol pa, c15h26o, an active chemical ingredient reputed for free radical scavenging and antiinflammatory properties, was investigated for its antiphotoaging action using a mouse model whose dorsal skin was depilated. Protective effects of patchouli alcohol isolated from. Chemical compound characterizations of patchouli leaf extract via gcms, lcqtofms, ftir, and 1h nmr written by sangeethavani sundarajan, nour hamid abdurahman published on 20190701 download full article with reference data and citations. Characterization of pa was performed by melting point mp, infrared spectroscopy ir, 1h and c nuclear magnetic resonance spectroscopy. Antidepressant activity of patchouli alcohol microcapsule. Availability, pharmaceutics, security, pharmacokinetics, and. Cd was prepared to form the inclusion complex with a saturated aqueous solution method, which was designed to improve the solubility and stability of pa. Nowadays, pa has been widely used in chemicals, flavors, and perfumery industry. Prevention of uv radiationinduced cutaneous photoaging in mice by topical. Influenza virus, known to cause flu, that is extremely hard to treat or cure, can be stopped from replicating, by treatment with patchouli oil. Patchouli oil isolated from the leaves of pogostemon. Prediction of chemical content in patchouli oil pogostemon cablin. The dorsal skin areas of sixweekold mice were smeared with pa solution or vehicle, followed by uv irradiation for nine consecutive weeks. In this study, patchouli alcohol pa, c 15 h 26 o, an active chemical ingredient reputed for free radical scavenging and antiinflammatory properties, was investigated for its antiphotoaging action using a mouse model whose dorsal skin was depilated.
Chemical compound characterizations of patchouli leaf. As expected on mechanistic considerations,32 the spectrum clearly indicates a dlactone normal value ann 250 nyc f. All mass spectra in this site plus many more are available from the nistepanih mass spectral library. Alpha copaene is required in sni 0623852006 wth max 0,5%, it means the patchouli oil after purification fulfilled sni 0623852006. Patchouli alcohol is a carbotricyclic compound and sesquiterpenoid tertiary alcohol that is tricyclo5. The uv spectra of two solvents, a simple alcohol and a simple ketone, are shown. The essential oil is a sensitive and susceptive material towards high temperature, oxidation, uv light, and humidity which are. Top, mass spectrum electron ionization, references data from nist standard reference database 69. Identification of patchouli chemical properties on oil. I am trying to purify patchauli alcohol from patchouli oil. Efforts to purify patchouli alcohol from patchouli oil pogostemon cablin. Characterisation of a recombinant patchoulol synthase.
Exposure to uv irradiation is also associated with an oxidative stress response due to the accumulation of reactive oxygen spices ros. Yutaka inoue 1 nana komiya 1 isamu murata 1 shunichi mitomo 2 yukiko negishi 2 ikuo kanamoto 1. Solubility phase analysis was performed to forecast the enhanced solubility of pa by. Prevention of uv radiationinduced cutaneous photoaging in mice by topical administration of patchouli oil. Patchouli alcohol significantly accelerates the recovery of the uv induced skin lesions, evidently through antioxidant and antiinflammatory action, as well as downregulation of the mmp1 and mmp3 expression. Analysis of patchouli alcohol by hplc using coreshell column. Patchoulol is also used in the synthesis of the chemotherapy drug taxol. A pharmacokinetic study of patchouli alcohol after a single oral. Led by mark pimentel, md, the research team of the medically associated science and technology mast program at cedarssinai has been developing the patentpending healight platform since 2016 and has produced a growing body of scientific evidence demonstrating preclinical safety and effectiveness of the technology as an antiviral and antibacterial treatment. Therefore, patchouli alcohol could be used as a marker for quality control of patchouli oils and the extracts.
We leverage our wide spectrum of business in the fields of development, manufacturing, marketing, and distribution to help you make bestinformed decisions tailored to your evolving needs for premium chemicals. Patchouli alcohol pa, the chemical structure is shown in figure 1, is a tricyclic sesquiterpene with a distinct odor of patchouli like earthy and camphor. Let us continue reading to know about some of the best health benefits of patchouli oil. In most studies, active compounds were extracted from ginger by aqueousethanol or some. Patchoulol or patchouli alcohol c 15 h 26 o is a sesquiterpene alcohol found in patchouli. Marine drugs free fulltext the protective effect of. Development of patchouli extraction with quality control. Topical application of patchouli alcohol inhibited uv induced reactive oxygen species that led to photoaging in mouse skin feng et al. It has antibacterial, antifungal, and antiviral actions. Patchouli alcohol pa is a tricyclic sesquiterpene extracted from pogostemonis herba, which is a traditional chinese medicine used for therapy of inflammatory diseases. Patchouli alcohol pa, the major active sesquiterpene found in pogostemonis herba, is largely responsible for pharmacological activities of this herb. Ultraviolet uv irradiation, known to generate reactive oxygen species ros excessively and elicit inflammatory response, is a potent inducer for skin photoaging. Evaluation of the antibacterial activity of patchouli oil ncbi. Patchouli essential oil, featured in perfumes, soaps, cosmetics, and as incense, is used by aromatherapists for its calming and reviving effects.
Patchouli alcohol pa, a tricyclic sesquiterpene, is one of the critical bioactive. Doubly deuteriumlabeled patchouli alcohol from cyclization of singly labeled 22h 1farnesyl diphosphate catalyzed by recombinant patchoulol synthase. Patchouli alcohol is an essential oil compound which obtained by fractional distillation of patchouli oil pogostemon cablin benth. Particularly, pogostone and patchouli alcohol have potent antimicrobial activity. Potential covid19 treatment thru uv light does exist as. Alcohols are the most therapeutically beneficial type of component in an essential oil, and have a low toxicity for safer. Patchouli alcohol pa is a tricyclic sesquiterpene isolated from pogostemon cablin, which exerts antiinflammatory, antiinfluenza and cognitiveenhancing bioactivities. Patchouli alcohol is the main component of patchouli oil. The uv spectra of two solvents, a simple alcohol and a.
A total of five constituents from introduced variety and nine constituents from local variety which represent over 90% of the oil constituents were identified according to mass spectrum of each constituent. Please see the following for information about the library and its accompanying search program. Patchouli alcohol c15h26o, a naturally occurring tricyclic sesquiterpene, is the critically biological active constituent among the patchouli oil extracted from patchouli and usually used as a pivotal chemical marker compound for the assessments in quality control of pogostemonis herba and patchouli oil in china 11. It is a tertiary alcohol, a sesquiterpenoid and a carbotricyclic compound.
Coates contribution from department of chemistry, university of illinois, 600 south mathews avenue, urbana, il 61801. Table 1 showed that patchouli oil purification is increased alpha copaene and patchouli alcohol. The present study aimed to investigate the protective effects of pa on acute lung injury ali induced by intratracheal instillation of lipopolysaccharide lps in mice. Comparative analysis of chemical components of purified. Patchouli oil po is a sort of food supplement with high medicinal value. It is used as a fragrance ingredient with a consumption of 0. This study was designed to examine if patchouli alcohol affects adipogenesis, and investigates the underlying mechanisms whereby patchouli alcohol exerts antiobesity effect. The ir spectrum initially assigned to patchoulene 23 isolated from peo. The acetic acidinduced writhing test and formalininduced plantar injection test in mice were employed to confirm the effect in vivo. Solvent cut time 3 min, range of mass spectrum 400700 mz. This essential oil is also characterized by a large number of other sesquiterpene hydrocarbons such as. To explore the antinociceptive effect of patchouli alcohol pa, the essential oil isolated from pogostemon cablin blanco bent, and determine the mechanism in molecular levels. Three statistical experimental designs for enhancing yield of active compounds from herbal medicines and antimotion sickness bioactivity. However, the commercial value of patchouli oil depends on the.
Using a variety of tests, they assessed the potential protective effects of patchouli oil. Screening of local and introduced varieties of pogostemon. Patchouli oil is an important material in perfumery. It can also be used as a precursor to some esters used in perfumes, soaps and in the flavor industries. Patchouli alcohol significantly accelerates the recovery of the uvinduced skin lesions, evidently through antioxidant and antiinflammatory action, as well as down. It caused by the absorbing of waste component and color substance then fe content by bentonite absorbent. Synthesis of patchouli alcohol journal of the american chemical. Isolation of patchouli alcohol from patchouli oil by. Patchouli alcohol is useful because of its antiviral properties and may also treat herpes virus infections in.
Author links open overlay panel xuexuan feng a 1 xiuting yu b 1 wenjie li a songzhi kong a yuhong liu a xie zhang a yanfang xian c xiaojun zhang a ziren su a zhixiu lin c. Three statistical experimental designs for enhancing yield. The mass spectrum plot was applied using a fullscan monitoring mode with a mass scan. Intracellular calcium ion was imaged to verify pa on muopioid receptor mor. The spectra of bentonite the main absorption peakabsorption on bentonite at. Appreciate your suggestion of column and gradient conditions. Quantitative determination of patchouli alcohol in the patchoulil oils and the hexane extract was performed by gcms using the new optimum conditions. Effects of topical application of patchouli alcohol on the uv induced skin photoaging in mice. Patchouli oil may also help in preventing and treating viral infections. Nist chemistry webbook the national institute of standards and technology nist uses its best efforts to deliver a high quality copy of the database and to verify that the data contained therein have been selected on the basis of sound. Effects of topical application of patchouli alcohol on the uv induced skin.
The absorption of wavelength on the chemical compound of a material on a transflectan. Spectrum chemical has exactly the form and grade of benzyl alcohol for your lab needs. Investigation of inclusion complex of patchouli alcohol. Some say you either love or hate the smell of patchouli. Thus, patchouli leaf oil is an important plant endowed with different bioactive compounds.
The patchoulol synthase pts from pogostemon cablin indian patchouli is a. The essential oil has also been shown to impart antioxidant activity 5. Patchouli alcohol compound information and applications for gc gas chromatography and lc liquid chromatography analysis. Patchouli alcohol is a sesquiterpene alcohol found in pogostemon cablin and possesses health benefit activities. Patchouli alcohol has several benefits in aromatheraphy field.
Patchouli alcohol is useful for reduce depression by breathing is steam. Patchouli patchouli essential oil patchouli oil uses. In view of this and the abnormally low uv absorption31 of the compound, structure xix appeared unreliable, a contention which was proved by a study of its pmr spectrum fig. Purification results with uv vis spectrophotometer showed that the activation energy at a.
Excessive alcohol consumption can cause serious hepatic injury which is associated with oxidative stress and fatty metabolic disturbance. These results indicate that leaves have a potential broad spectrum antibacterial activity. This included cells growing in a petri dishlike setting, as well as using the cells to grow actual tumors in living mice, which are known as xenografts. Fourier transformationinfrared ftir spectroscopy, powder xray diffraction. Why you should train your nose to make patchouli palatable.
Pdf inhibition effects of patchouli alcohol against. The main active ingredient of the oil, patchouli alcohol, was tested on human lung cancer models by scientists in shanghai. Pdf isolation of patchouli alcohol from patchouli oil by. Pdf purification and analysis of patchouli alcohol from patchouli. The results showed that patchouli oil contained more patchouli alcohol than the. Meanwhile, ultraviolet b uvb, shorter wavelength ultraviolet radiations mainly affects the skins epidermal layers, causing erythema, edema, and wrinkles 3,4. Patchouli alcohol significantly accelerates the recovery of the uv induced skin lesions, evidently through antioxidant and antiinflammatory action, as well as downregulation of the mmp1. Benzyl alcohol, also known as phenylcarbinol, is an aromatic alcohol used as a solvent due to its polarity, low vapor pressure, and low toxicity.